Signalling

Part:BBa_K1718005:Design

Designed by: Michael Donovan   Group: iGEM15_CU_Boulder   (2015-09-15)


LuxI and GFP behind a Bxb1 compatible genetic switch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 55
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 962
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 64
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 84
    Illegal BsaI.rc site found at 245
    Illegal BsaI.rc site found at 1638


Design Notes

When on a high copy plasmid (pSB1C3), there was some leaky expression; therefore, we recommend that this part be used on plasmids with a lower copy number.


Source

The luxI and GFP come from the distribution kit. See BBa_K1718002 for more details on the switch.

References